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Defocustracker without calibration/background images?
#1 · May 27, 2022, 1:09 pm
Hello!
So, in the walkthrough examples, the training data was used to build the model.
But I have images where the particles are all in the same focal plane, so no z-calculations needed.
How can I run defocustracker in this case? (The images are brightfield and very noisy, so I need to get rid of the background noise, but can I try it without having background noise images without particles in them?